Brain Sketch

NEUROENDOCRINE MODULATORS AND ISCHEMIC STROKE

Ongoing

The stroke field is a relatively new avenue for the Ashpole laboratory. We previously found that reducing IGF-1R in astrocytes alters their ability to uptake glutamate, a major contributor to ongoing stroke damage. Furthermore, there is a significant amount of evidence showing that low IGF-1 is associated with poor stroke outcomes and increased mortality. In our literature review (currently under review), we highlight evidence in preclinical models that show administering exogenous IGF-1 in the hours surrounding stroke attenuates neurological deficits and improves behavioral outcomes. Thus, we aim to understand the underlying cellular mechanisms involved in the previously observed protection from administering IGF-1 in stroke models. This project is currently ongoing, so subscribe for updates!

This project is currently funded by the University of Mississippi Graduate Research Counsel Grant.

Techniques:

Transgenic Mouse Models

Middle Cerebral Artery Occlusion

TTC Stain

Sensorimotor Behavioral Test

Immunohistochemistry

Western Blot

Cell Culture

Confocal Microscopy